HPLC Column, NP Amino, Wide Pore, 10um, 4.0mm ID x 100mm Length, 300A. Cogent Brand. 1 EA.
Additional Information:
Cogent NP Amino Wide Pore is a versatile normal phase and HILIC stationary phase designed for retention of highly polar compounds and larger molecules that are often difficult to retain using conventional reversed-phase columns. Manufactured on high-purity 300 Å wide pore silica with a propyl amino bonded phase, it provides excellent selectivity for complex carbohydrates, sugars, organic acids, and other highly polar analytes. This stationary phase is commonly used as an alternative for USP L8 methods and is suitable for both Normal Phase and HILIC separations involving larger molecular species.
68040-10P-4-3M is an analytical HPLC column packed with 10 µm NP Amino Wide Pore particles featuring a 300 Å pore size in a 4.0 mm ID x 100 mm format. The 100 mm length provides increased resolution compared to shorter columns while maintaining efficient analysis times. The 10 µm particle size supports higher flow rates with lower operating backpressure, while the wide pore structure improves accessibility for larger molecules. Typical operating flow rates are approximately 0.8–1.5 mL/min depending on analyte properties, mobile phase composition, and instrument configuration.
This column is particularly effective for complex sugars, carbohydrates, negatively charged compounds, organic acids, carboxylic acid-containing compounds, and other highly polar molecules. The wide pore design provides improved performance for larger analytes in both Normal Phase and HILIC applications. As with all amino phases, this column is suitable for HILIC applications when enhanced retention of polar compounds is required.
Key Benefits
NP Amino Wide Pore stationary phase
USP L8 equivalent chemistry
Propyl amino bonded phase
300 Å wide pore silica
Suitable for Normal Phase chromatography
Suitable for HILIC applications
Enhanced retention of polar compounds
Improved accessibility for larger molecules
Low operating backpressure with 10 µm particles
Useful for method development and preparative scouting
Typical Applications
USP L8 methods
HILIC separations
Normal Phase chromatography
Complex sugar analysis
Carbohydrate analysis
Organic acid analysis
Carboxylic acid analysis
Food and beverage testing
Pharmaceutical analysis
Preparative method scouting
Specifications
Product Type: HPLC Column
Stationary Phase: NP Amino Wide Pore
USP Classification: L8
Chromatography Mode: Normal Phase / HILIC
Bonded Phase: Propyl Amino
Endcapping: Not Endcapped
Particle Size: 10 µm
Pore Size: 300 Å
Column Dimensions: 4.0 mm ID x 100 mm
Length: 100 mm
Typical Flow Rate: 0.8–1.5 mL/min
Brand: Cogent
Special Order Item Information
Important Notes
Packed and shipped in Normal Phase solvents
Suitable for both Normal Phase and HILIC chromatographic modes
Wide pore design improves access for larger molecules and complex carbohydrates
Excellent retention of sugars, carbohydrates, and organic acids
Increased column length provides improved resolution compared to shorter formats
Suitable for method development and preparative chromatography scouting
Special Order Item